Leishmania major strain Friendlin, phosphatase 2c, putative

Target (protein/gene name): phosphatase 2c, putative
*NCBI Gene:XP_001685487.1
*Protein ID: 1A6Q, LmjF30.0380

*Organism (including strain): Leishmania major strain Friedlin

Etiologic Risk Group (see link below):
Leishmania is a protozoan and its parasite reposible for the disease leishmaniasis. It is spread to humans though bloodsucking sand flies. The symptoms of leishmaniasis are skin sores, which erupt weeks to months after a person is affected. The infection include fever, damage to the spleen and liver and anemia.

Essentiality of this protein:
LmjF30.0380 has essentiality data

Gene/Ortholog: Tb927.6.1800 (OG4_19717); Phenotype: significant loss of fitness in bloodstream forms (3 days); Source study: alsford

Gene/Ortholog: Tb927.6.1800 (OG4_19717); Phenotype: no significant loss or gain of fitness in bloodstream forms (6 days); Source study: alsford

Gene/Ortholog: Tb927.6.1800 (OG4_19717); Phenotype: no significant loss or gain of fitness in procyclic forms; Source study: alsford

Gene/Ortholog: Tb927.6.1800 (OG4_19717); Phenotype: significant loss of fitness in differentiation of procyclic to bloodstream forms; Source study: alsford

Complex of proteins?:1
Druggable Target:yes

*EC#:3.1.3.16
Link to BRENDA EC# page: http://www.brenda-enzymes.org/php/result_flat.php4?ecno=3.1.3.16


Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):



http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Enzyme_Assay/calcineurin.pdf



http://iai.asm.org/content/75/5/2136

http://www.biomedcentral.com/1471-2164/8/434

http://www.ncbi.nlm.nih.gov/pubmed/10722732



-- List cost and quantity of substrate reagents and supplier



Structure Available (PDB or Homology model)

http://www.pdb.org/pdb/explore/explore.do?pdbId=1a6q



PDB#: 1A6Q



Current Inhibitors: none



Expression Information (has it been expressed in bacterial cells): Escherichia coli





Purification Method:Purification is accomplished by ammonium sulfate fractionation, Sephadex G-75 chromatography, affinity chromatography on Amicon Matrex Gel Orange A and chromatofocusing.



Image of protein (PyMol with features delineated and shown separately):



amino acid sequence:



MGLMLPKPILSKVVDRAGNSFVNAACASQNGFRNSMEDAHMLVATDDADVAYFGIFDGHSNAECSAYVARELPQRLKKLPESITAEMLEKVCIEVDEAYMKTSTEGGTTGTFCVIRKDLTVTIANVGDSRILVCRGGKLIFATEDHKPYIPGETERIVACGGSVVSNRVDGDLAVSRAFGDASFKVKGTKDYRMQKVIAVPDVSVLQCEPNDFIILACDGVFEGNFSNEDVCQFVWEQQQNCWDDLAVVACRVCDEAIRCGSKDNISCLVVQLAEGASRVKAFGATSFVPGPPFPRNQQPCRAAYAQMAELGHTTTAAALQTRYQLLQAFSKNKLNSQPPVMRTAFEMSDEVDVETEWSFFGKGPAPGNEKNFFEALANTGNSS


Number of amino acids: 384



Molecular weight: 41732.2



Theoretical pI: 4.97

atgggcctta tgctaccgaa accgatcctt agcaaggtcg tggatcgtgc agggaactct tttgtaaacg ctgcctgcgc ctcgcagaac ggctttcgca actccatgga ggatgcacac atgttagtcg ccaccgacga cgccgacgtg gcgtactttg gcatcttcga tgggcatagt aacgccgagt gtagcgccta tgtggcgagg gagctgccac agcggctgaa gaagctgccg gagtcgatca cggcggagat gttagagaag gtatgcatag aggtggatga ggcctacatg aagaccagca ccgagggcgg caccaccggc accttttgcg tcatccgcaa ggacctcaca gtgaccatcg ccaacgtcgg tgactcccgc atcctcgtct gccgcggcgg caaactgatt ttcgccactg aagaccacaa gccgtacatt ccgggggaga cggagcgcat cgttgcctgc ggcggcagtg tagtcagcaa ccgcgtcgac ggagaccttg ctgtatcgcg cgcctttggt gatgcgtcct tcaaggtaaa ggggacaaag gactaccgaa tgcagaaggt gattgccgtg cccgacgtgt cggtgctgca atgcgagccg aacgacttta tcattctcgc ctgtgacggc gtcttcgagg gcaacttctc caacgaggac gtttgccagt ttgtgtggga gcagcagcaa aactgctggg acgacttggc ggttgtggcg tgccgcgttt gcgatgaggc gatccggtgc ggcagcaagg acaacatctc ctgcttagtg gtgcagctgg ccgagggcgc ttccagggta aaggcattcg gcgccacatc cttcgtcccg gggccgccct ttccgcgcaa tcagcagccc tgccgcgccg cctacgcgca gatggcagag ctcggccaca ccacgacggc ggcagcgctg cagacgcgct accagcttct gcaggcgttc tccaagaaca agttgaattc gcagccgcct gtgatgcgaa ccgctttcga gatgagcgac gaggtggacg tggagacgga atggtccttc ttcggcaagg gccccgcgcc aggaaacgag aagaacttct ttgaggcgct cgccaatacg ggcaacagct cgtag

*GC% Content for gene:

*CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):

*GC% Content for gene (codon optimized):

Resources:
See ProtocolTargetDiscoveryVDS.docx for more

Etiologic Risk Group Categories (for pathogens):http://www.utexas.edu/research/rsc/ibc/agent_class.html#_Toc7238334



Databases of genes/organisms:

http://www.niaid.nih.gov/Pages/default.aspx

http://eupathdb.org/eupathdb/

https://patricbrc.vbi.vt.edu/portal/portal/patric/Home

http://www.nmpdr.org/FIG/wiki/view.cgi/Main/EssentialGenes

http://tubic.tju.edu.cn/deg/

http://csgid.org/csgid/cake/pages/community_request_gateway

http://tdrtargets.org/

http://gsc.jcvi.org/status.shtml